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cas9 protein  (New England Biolabs)


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    Structured Review

    New England Biolabs cas9 protein
    Cas9 Protein, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cas9+protein/EnGen+Spy+dCas9/pm42126097-349-7-12
    Average 98 stars, based on 59 article reviews
    cas9 protein - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Injection:

    Article Title: Mytho/Phaf1 is required to prevent DNA damage and tissue degeneration in Danio rerio.
    Article Snippet: The resulting template was transcribed in vitro using the SP6 transcription kit (MEGAscript SP6 kit, AM1330, ThermoFisher Scientific, Monza, Italy), adhering to the manufacturer's guidelines. .. At the 1-cell stage, embryos were injected with 1 nl of a solution containing 280 ng/μL of Cas9 protein (M0646, New England Biolabs, Milan, Italy) and 100 ng/μL of sgRNA, with 0.05% phenol red as an injection tracer. ..

    Article Title: PGC-derived migrasomes couple PGC proliferation with migration.
    Article Snippet: .. To generate F0 CRISPR/Cas9-mediated mutagenesis of acvr1ba, embryos at the one-cell stage were injected with a mixture of three acvr1ba-specific gRNAs together with Cas9 protein (NEB, M0646T). ..

    Article Title: METTL18 ensures pancreatic function by maintaining proper translation and proteostasis
    Article Snippet: CRISPR RNA (crRNA) oligos for upstream intron (5′- AAAGGCGTCTAAAACGTCTT-3′) and downstream exon2 (5′- CAACGGTTTGGTGATCGATG-3′) were purchased from FASMAC (Kanagawa, JAPAN). .. The crRNAs, Cas9 protein (NEB, #M0646T) and tracrRNA (Fasmac) were injected into fertilized eggs (mouse strain C57BL/6J), and the mutated Mettl18 gene versions were screened by PCR. ..

    Article Title: A quantitative in vivo CRISPR-imaging platform identifies regulators of hyperplastic and hypertrophic adipose morphology in zebrafish
    Article Snippet: Pooled in vitro transcription of gRNAs was performed as per , and RNA was cleaned using Zymoclean columns (Zymo Research, Cat. # R1013). .. Prior to injection, gRNAs were incubated with Cas9 protein (New England Biolabs, Cat. # M0646T) and heated for 5 min at 37°C. .. Mutagenesis of individual gRNAs was verified using T7E1 assays (New England Biolabs, Cat. # M0302S). gRNAs were injected into zebrafish embryos at the one-cell stage as per , along with a Fast Green dye to screen for successfully injected embryos.

    Magnetic Beads:

    Article Title: Prospects of multipurpose biomonitoring for fisheries assessment based on environmental nucleic acids.
    Article Snippet: .. Another strategy would be using a deactivated Cas9 protein (EnGen® Spy dCas9, New England Biolabs) that binds to the target thanks to the gRNA and can be labelled with biotin so the target fraction is selected with magnetic beads and followed by a proteinase K treatment to release the dsDNA (Liu et al., 2017; Slesarev et al., 2019). ..

    CRISPR:

    Article Title: PGC-derived migrasomes couple PGC proliferation with migration.
    Article Snippet: .. To generate F0 CRISPR/Cas9-mediated mutagenesis of acvr1ba, embryos at the one-cell stage were injected with a mixture of three acvr1ba-specific gRNAs together with Cas9 protein (NEB, M0646T). ..

    Article Title: ACRC/GCNA is an essential protease that repairs DNA-protein crosslinks during vertebrate development.
    Article Snippet: .. Creation of mutant zebrafish strains using CRISPR/Cas9 system, genotyping Genetically modified zebrafish lines were created using Cas9 protein (EnGen ® Spy Cas9 NLS, #M0646T, NEB) and previously established protocols combining PCR and reverse transcription to generate guide RNAs [ 48 ]. ..

    Mutagenesis:

    Article Title: PGC-derived migrasomes couple PGC proliferation with migration.
    Article Snippet: .. To generate F0 CRISPR/Cas9-mediated mutagenesis of acvr1ba, embryos at the one-cell stage were injected with a mixture of three acvr1ba-specific gRNAs together with Cas9 protein (NEB, M0646T). ..

    Article Title: ACRC/GCNA is an essential protease that repairs DNA-protein crosslinks during vertebrate development.
    Article Snippet: .. Creation of mutant zebrafish strains using CRISPR/Cas9 system, genotyping Genetically modified zebrafish lines were created using Cas9 protein (EnGen ® Spy Cas9 NLS, #M0646T, NEB) and previously established protocols combining PCR and reverse transcription to generate guide RNAs [ 48 ]. ..

    Polymerase Chain Reaction:

    Article Title: METTL18 ensures pancreatic function by maintaining proper translation and proteostasis
    Article Snippet: CRISPR RNA (crRNA) oligos for upstream intron (5′- AAAGGCGTCTAAAACGTCTT-3′) and downstream exon2 (5′- CAACGGTTTGGTGATCGATG-3′) were purchased from FASMAC (Kanagawa, JAPAN). .. The crRNAs, Cas9 protein (NEB, #M0646T) and tracrRNA (Fasmac) were injected into fertilized eggs (mouse strain C57BL/6J), and the mutated Mettl18 gene versions were screened by PCR. ..

    Article Title: ACRC/GCNA is an essential protease that repairs DNA–protein crosslinks during vertebrate development
    Article Snippet: .. Genetically modified zebrafish lines were created using Cas9 protein (EnGen ® Spy Cas9 NLS, #M0646T, NEB) and previously established protocols combining PCR and reverse transcription to generate guide RNAs [ ]. ..

    Article Title: ACRC/GCNA is an essential protease that repairs DNA-protein crosslinks during vertebrate development.
    Article Snippet: .. Creation of mutant zebrafish strains using CRISPR/Cas9 system, genotyping Genetically modified zebrafish lines were created using Cas9 protein (EnGen ® Spy Cas9 NLS, #M0646T, NEB) and previously established protocols combining PCR and reverse transcription to generate guide RNAs [ 48 ]. ..

    Genetically Modified:

    Article Title: ACRC/GCNA is an essential protease that repairs DNA–protein crosslinks during vertebrate development
    Article Snippet: .. Genetically modified zebrafish lines were created using Cas9 protein (EnGen ® Spy Cas9 NLS, #M0646T, NEB) and previously established protocols combining PCR and reverse transcription to generate guide RNAs [ ]. ..

    Article Title: ACRC/GCNA is an essential protease that repairs DNA-protein crosslinks during vertebrate development.
    Article Snippet: .. Creation of mutant zebrafish strains using CRISPR/Cas9 system, genotyping Genetically modified zebrafish lines were created using Cas9 protein (EnGen ® Spy Cas9 NLS, #M0646T, NEB) and previously established protocols combining PCR and reverse transcription to generate guide RNAs [ 48 ]. ..

    Reverse Transcription:

    Article Title: ACRC/GCNA is an essential protease that repairs DNA–protein crosslinks during vertebrate development
    Article Snippet: .. Genetically modified zebrafish lines were created using Cas9 protein (EnGen ® Spy Cas9 NLS, #M0646T, NEB) and previously established protocols combining PCR and reverse transcription to generate guide RNAs [ ]. ..

    Article Title: ACRC/GCNA is an essential protease that repairs DNA-protein crosslinks during vertebrate development.
    Article Snippet: .. Creation of mutant zebrafish strains using CRISPR/Cas9 system, genotyping Genetically modified zebrafish lines were created using Cas9 protein (EnGen ® Spy Cas9 NLS, #M0646T, NEB) and previously established protocols combining PCR and reverse transcription to generate guide RNAs [ 48 ]. ..

    Incubation:

    Article Title: A quantitative in vivo CRISPR-imaging platform identifies regulators of hyperplastic and hypertrophic adipose morphology in zebrafish
    Article Snippet: Pooled in vitro transcription of gRNAs was performed as per , and RNA was cleaned using Zymoclean columns (Zymo Research, Cat. # R1013). .. Prior to injection, gRNAs were incubated with Cas9 protein (New England Biolabs, Cat. # M0646T) and heated for 5 min at 37°C. .. Mutagenesis of individual gRNAs was verified using T7E1 assays (New England Biolabs, Cat. # M0302S). gRNAs were injected into zebrafish embryos at the one-cell stage as per , along with a Fast Green dye to screen for successfully injected embryos.



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